Problem
Firstly, all your operations should be carried out under sterile conditions and on ice to prevent cell contamination and damage. The temperature and time of heat shock need to be strictly controlled. Generally, a heat shock of 42 ℃ for 60 seconds is a commonly used condition. The conversion efficiency of SOC medium is twice that of LB medium, so we recommend using SOC medium for operation. Finally, it is recommended to place the agar plate and preheat it in the incubator for half an hour, which is beneficial for coating and can quickly grow colonies.