DH5α Competent Cells are readymade chemoreceptor cells for conventional plasmid cloning, which are α-complementary to the amino end of β-galactosidase encoded by the pUC series plasmid vector, and can be used to screen for blue-white spot identification of recombinant strains.
Contact UsThe English name is DH5 α Competitive Cells, which is a ready to use chemocompetent cell used for conventional plasmid cloning. When transformed using pUC series plasmid vectors, it can achieve α - complementarity with the amino terminus of β - galactosidase encoded by the vector, and can be used for screening and identifying recombinant strains in blue and white spots.
instructions:
Remove the sensory cells from the -80 ° C freezer and quickly place them on ice. When they have just melted, add the DNA sample (or ligation product) and gently mix. Ice bath for 30 minutes; Heat shock in a 42 ° C water bath for 90 seconds, then quickly ice bath for 2-3 minutes without shaking during this period; Add 600ul of sterile medium without antibiotics, shake at 37 ℃ for 1 hour (180-230rpm), draw an appropriate volume and evenly apply it to the corresponding resistant plate, invert at 37 ℃ overnight for cultivation.
Storage conditions:
Long term storage at -80 ° C in a refrigerator can maintain stability for six months and avoid repeated freezing and thawing.
1. This product is only used for scientific research by professionals and should not be used in clinical practice, food and medicine, nor should it be stored in public areas such as home environments.
2. For your safety and health, please wear lab coats and disposable gloves when operating.
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