The English name is BL21 Competitive Cells, and a series of prokaryotic expression strains such as BL21 (DE3), Rosetta, OrigamiB (DE3), etc. are all derived from the BL21 strain. This strain is mainly used for the expression of non-toxic proteins and does not contain T7 RNA polymerase, so it cannot be used for protein expression driven by the T7 promoter. However, it contains E. coli RNA polymerase.
Contact UsThe English name is BL21 Competitive Cells, and a series of prokaryotic expression strains such as BL21 (DE3), Rosetta, OrigamiB (DE3), etc. are all derived from the BL21 strain. This strain is mainly used for the expression of non-toxic proteins and does not contain T7 RNA polymerase, so it cannot be used for protein expression driven by T7 promoter (such as pET series); But it contains E. coli RNA polymerase, which can be used for the expression of prokaryotic systems such as tac or trc that use E. coli RNA polymerase, such as: pGEX, pMAL。 BL21 competent cells are used as hosts for expression vectors. Suitable for the expression of toxic proteins and the Tac promoter system, such as: pGEX， pMAL。
Storage conditions:
Long term storage at -80 ° C in a refrigerator can maintain stability for six months and avoid repeated freezing and thawing.
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